Healthcare Facilities as Potential Reservoirs of Antimicrobial Resistant Klebsiella pneumoniae: An Emerging Concern to Public Health in Bangladesh

The emergence of virulent extended spectrum beta-lactamase producing Klebsiella pneumoniae (ESBL-KP) including carbapenem-resistant Klebsiella pneumoniae (CRKP) in hospital-acquired infections has resulted in significant morbidity and mortality worldwide. We investigated the antibiotic resistance and virulence factors associated with ESBL-KP and CRKP in tertiary care hospitals in Bangladesh and explored their ability to form biofilm. A total of 67 ESBL-KP were isolated from 285 Klebsiella pneumoniae isolates from environmental and patient samples from January 2019 to April 2019. For ESBL-KP isolates, molecular typing was carried out using enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR), antibiotic susceptibility testing, PCR for virulence and drug-resistant genes, and biofilm assays were also performed. All 67 isolates were multidrug-resistant (MDR) to different antibiotics at high levels and 42 isolates were also carbapenem-resistant. The most common beta-lactam resistance gene was bla(CTX-M-1) (91%), followed by bla(TEM) (76.1%), bla(SHV )(68.7%), bla(OXA-1) (29.9%), bla(GES) (14.9%), bla(CTX-M-9) (11.9%), and bla(CTX-M-2) (4.5%). The carbapenemase genes bla(KPC) (55.2%), bla(IMP) (28.4%), bla(VIM) (14.9%), bla(NDM-1) (13.4%), and bla(OXA-48) (10.4%) and virulence-associated genes such as fimH (71.6%), ugeF (58.2%), wabG (56.7%), ureA (47.8%) and kfuBC (28.4%) were also detected. About 96.2% of the environmental and 100% of the patient isolates were able to form biofilms. ERIC-PCR-based genotyping and hierarchical clustering of K. pneumoniae isolates revealed an association between environmental and patient samples, indicating clonal association with possible transmission of antimicrobial resistance genes. Our findings can help in improving patient care and infection control, and the development of public health policies related to hospital-acquired infections.

Automated summary

The emergence of virulent extended spectrum beta-lactamase producing Klebsiella pneumoniae (ESBL-KP) including carbapenem-resistant Klebsiella pneumoniae (CRKP) in hospital-acquired infections has led to significant morbidity and mortality worldwide. A study conducted in tertiary care hospitals in Bangladesh revealed that these bacterial strains exhibited high levels of antibiotic resistance, could form biofilms, and contained various antibiotic resistance and virulence genes. The findings are important for improving patient care, infection control, and the development of public health policies.