期刊
JOURNAL OF PERSONALIZED MEDICINE
卷 12, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/jpm12101577
关键词
wound repair; airway epithelial cells; CFTR; cystic fibrosis; CFTR modulators
资金
- University of Foggia [392-2019, 13546-VII/1, 01/04/2019]
The novel triple combination therapy ETI shows promise in ameliorating wound healing of airway epithelium in patients with the F508del mutation.
Background: Cystic fibrosis (CF) airway epithelium shows alterations in repair following damage. In vitro studies showed that lumacaftor/ivacaftor (Orkambi) may favor airway epithelial integrity in CF patients. Our aim was to evaluate the effect of the novel triple combination elexacaftor/tezacaftor/ivacaftor (ETI) on wound repair in CF airway epithelial cells. Methods: A tip-based scratch assay was employed to study wound repair in monolayers of CFBE14o- cells overexpressing the F508del mutation. ETI was added during wound repair. Results: ETI efficiently rescued CFTR F508del maturation and activity, accelerated wound closure and increased wound healing rates of the injured CF cell monolayers. Conclusions: The triple corrector/potentiator combination ETI shows promise in ameliorating wound healing of the airway epithelium in F508del patients.
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