Urinary extracellular vesicles signature for diagnosis of kidney disease

Congenital disorders characterized by the quantitative and qualitative reduction in the number of functional nephrons are the primary cause of chronic kidney disease (CKD) in children. We aimed to describe the alteration of urinary extracellular vesicles (uEVs) associated with decreased renal function during childhood. By nanoparticle tracking analysis and quantitative proteomics, we identified different:ally expressed proteins in uEVs in bilateral renal hypoplasia, which is characterized by a congenitally reduced number of nephrons. This expression signature of uEVs reflected decreased renal function in CKD patients by congenital anomalies of the kidney and urinary tract or cilia pathy. As a proof-of-concept, we constructed a prototype ELISA system that enabled the isolation of uEVs and quantitation of expression of molecules representing the signature. The system identified decreased renal function even in its early stage. The uEVs signature could pave the way for non-invasive methods that can complement existing testing methods for diagnosing kidney diseases.

Automated summary

This study describes the changes in urinary extracellular vesicles associated with decreased renal function in children. Proteomic analysis identified differentially expressed proteins in the urine extracellular vesicles of patients with congenital renal hypoplasia, reflecting the decreased renal function. A prototype ELISA system was also developed to quantify the expression of molecules representing this signature, which could detect early stages of decreased renal function.