期刊
ISCIENCE
卷 25, 期 10, 页码 -出版社
CELL PRESS
DOI: 10.1016/j.isci.2022.105052
关键词
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资金
- Research Laboratory Center, Faculty of Medicine
- JSPS KAKENHI [JP22K08759, JP21K19537, JP20H03745, JP19K09051]
- Okinawa Science and Technology Innovation System Construction Project (OSTC)
- Japan Agency for Medical Research and Development (AMED) [JP22bm0104001]
- Institute for Animal Experiments, Faculty of Medicine, University of the Ryukyus
Human hepatocytes were transformed into induced pluripotent stem cells (iPSCs) using Sendai virus vectors. The iPSCs expressed hepatocyte-specific markers and showed better efficiency in differentiating into hepatocyte-like cells compared to other types of iPSCs.
Human hepatocytes were transfected with Sendai virus vectors (SeV) express-ing OCT3/4, SOX2, KLF4, and C-MYC to produce hepatocyte-derived induced pluripotent stem cells (iPSCs). The messenger RNA (mRNA) expression of undifferentiated markers (passage 19-21) and hepatocyte-specific markers (HSMs) (passage 0-20) in 48 established hepatocyte-derived iPSC-like colonies was examined. Among the 48 clones, 10 clones continuously expressed HSM mRNA (HNF1 beta and HNF4 alpha) in passage 0-20. The colonies which expressed HSMs (iTS-L cells: induced tissue-specific stem cells from liver) showed a different tendency in microarray and methylation analyses to fibroblast-derived iPSCs (strain: 201B7). iTS-L cells were less likely to form teratomas in mice than iPSCs (He). The iTS-L cells were differentiated into hepatocyte-like cells more efficiently than iPSCs (He) or iPSCs (201B7). These data suggest that SeV ex-pressing OCT3/4, SOX2, KLF4, and C-MYC induce the generation of iPSCs and iTS-L cells.
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