期刊
JOURNAL OF MICROBIOLOGY
卷 55, 期 1, 页码 56-62出版社
MICROBIOLOGICAL SOCIETY KOREA
DOI: 10.1007/s12275-017-6581-6
关键词
Novosphingobium pentaromativorans; outer membrane vesicle; proteomics
类别
资金
- Korea Health Technology R&D Project through Korea Health Industry Development Institute (KHIDI)
- Ministry of Health & Welfare, Republic of Korea [HI-16C0950]
- Bio-Synergy Research Project of the Ministry of Science, ICT [NRF-2014M3A9C4066461]
- Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea [HI14C 726]
- Korea Health Promotion Institute [HI14C2726010016] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Novosphingobium pentaromativorans US6-1 is a Gram-negative halophilic marine bacterium able to utilize several poly cyclic aromatic hydrocarbons such as phenanthrene, pyrene, and benzo [a]pyrene. In this study, using transmission electron microscopy, we confirmed that N. pentaromativorans US6-1 produces outer membrane vesicles (OMVs). N. pentaromativorans OMVs (hereafter OMVNovo) are spherical in shape, and the average diameter of OMVNovo is 25-70 mn. Proteomic analysis revealed that outer membrane proteins and periplasmic proteins of N. pentaromativorans are the major protein components of OMVNovo. Comparative proteomic analysis with the membrane-associated protein fraction and correlation analysis demonstrated that the outer membrane proteins of OMVNovo originated from the membrane-associated protein fraction. To the best of our knowledge, this study is the first to characterize OMV purified from halophilic marine bacteria.
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