期刊
JOURNAL OF FUNGI
卷 8, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/jof8080887
关键词
Cordyceps militaris; genome-scale metabolic model; sphingolipid; metabolic footprinting; metabolic responses; transcriptome
资金
- Thailand Research Fund [RSA6180001]
- National Research Council of Thailand (NRCT)
- Office of the Ministry of Higher Education, Science, Research and Innovation
- Thailand Science Research and Innovation through the Kasetsart University Reinventing University Program
This study enhanced the genome-scale metabolic model of Cordyceps militaris and found key metabolic responses in alteration of lipid metabolism upon different carbon sources. The study provides a comprehensive basis for sphingolipid biosynthesis in Cordyceps militaris, which can be further applied in medicinal and functional food fields.
Cordyceps militaris is an industrially important fungus, which is often used in Asia as traditional medicine. There has been a published genome-scale metabolic model (GSMM) of C. militaris useful for predicting its growth behaviors; however, lipid metabolism, which plays a vital role in cellular functions, remains incomplete in the GSMM of C. militaris. A comprehensive study on C. militaris was thus performed by enhancing GSMM through integrative analysis of metabolic footprint and transcriptome data. Through the enhanced GSMM of C. militaris (called iPC1469), it contained 1469 genes, 1904 metabolic reactions and 1229 metabolites. After model evaluation, in silico growth simulation results agreed well with the experimental data of the fungal growths on different carbon sources. Beyond the model-driven integrative data analysis, interestingly, we found key metabolic responses in alteration of lipid metabolism in C. militaris upon different carbon sources. The sphingoid bases (e.g., sphinganine, sphingosine, and phytosphingosine) and ceramide were statistically significant accumulated in the xylose culture when compared with other cultures; this study suggests that the sphingolipid biosynthetic capability in C. militaris was dependent on the carbon source assimilated for cell growth; this finding provides a comprehensive basis for the sphingolipid biosynthesis in C. militaris that can help to further redesign its metabolic control for medicinal and functional food applications.
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