期刊
JOURNAL OF FUNGI
卷 8, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/jof8111117
关键词
shock waves; acoustic cavitation; gene expression; Aspergillus niger; cell permeabilization; fungal germination
资金
- CONACYT [372, CB-2017-2018-A1-S-21130]
This study investigated the effects of shock waves on Aspergillus niger and found that they did not significantly affect the viability and germination of conidia. However, increased aggressiveness of the treatment led to changes in certain genes and significant modifications to the cell wall. Under optimized conditions, shock waves could be used for various biotechnological applications.
Shock waves, as used in medicine, can induce cell permeabilization, genetically transforming filamentous fungi; however, little is known on the interaction of shock waves with the cell wall. Because of this, the selection of parameters has been empirical. We studied the influence of shock waves on the germination of Aspergillus niger, to understand their effect on the modulation of four genes related to the growth of conidia. Parameters were varied in the range reported in protocols for genetic transformation. Vials containing conidia in suspension were exposed to either 50, 100 or 200 single-pulse or tandem shock waves, with different peak pressures (approximately 42, 66 and 83 MPa). In the tandem mode, three delays were tested. To equalize the total energy, the number of tandem events was halved compared to the number of single-pulse shock waves. Our results demonstrate that shock waves do not generate severe cellular effects on the viability and germination of A. niger conidia. Nevertheless, increase in the aggressiveness of the treatment induced a modification in four tested genes. Scanning electron microscopy revealed significant changes to the cell wall of the conidia. Under optimized conditions, shock waves could be used for several biotechnological applications, surpassing conventional techniques.
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