期刊
BIOMEDICINES
卷 10, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/biomedicines10112718
关键词
extracellular vesicles; glioblastoma; meningioma; plasma; neurons; IgG; Fc; complement; C1q; apoptosis; neuroblastoma; cytotoxicity
资金
- National Institute of Health [NIMH1R21MH118174-01, 4R33MH118174]
- Department of Neurosurgery Research Funds
Glioblastoma extracellular vesicles (EVs) have dose-dependent cytotoxic effects on neurons and can induce IgG-mediated neuronal apoptosis and necrosis. GBM plasma EVs are smaller in size and their size does not correlate with concentration.
Glioblastoma (GBM) is the most aggressive and lethal form of brain tumor. Extracellular vesicles (EVs) released by tumor cells play a critical role in cellular communication in the tumor microenvironment promoting tumor progression and invasion. We hypothesized that GBM EVs possess unique characteristics which exert effects on endogenous CNS cells including neurons, producing dose-dependent neuronal cytotoxicity. We purified EVs from the plasma of 20 GBM patients, 20 meningioma patients, and 21 healthy controls, and characterized EV phenotypes by electron microscopy, nanoparticle tracking analysis, protein concentration, and proteomics. We evaluated GBM EV functions by determining their cytotoxicity in primary neurons and the neuroblastoma cell line SH-SY5Y. In addition, we determined levels of IgG antibodies in the plasma in GBM (n = 82), MMA (n = 83), and controls (non-tumor CNS disorders and healthy donors, n = 50) with capture ELISA. We discovered that GBM plasma EVs are smaller in size and had no relationship between size and concentration. Importantly, GBM EVs purified from both plasma and tumor cell lines produced IgG-mediated, complement-dependent apoptosis and necrosis in primary human neurons, mouse brain slices, and neuroblastoma cells. The unique phenotype of GBM EVs may contribute to its neuronal cytotoxicity, providing insight into its role in tumor pathogenesis.
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