Effects of the Exposure of Human Non-Tumour Cells to Sera of Pancreatic Cancer Patients

Pancreatic ductal adenocarcinoma (PDAC) has high metastatic potential. The genometastasis theory proposes that the blood of some cancer patients contains elements able to transform healthy cells by transferring oncogenes. Since findings on genometastasis in PDAC are still scarce, we sought supporting evidence by treating non-tumour HEK293T and hTERT-HPNE human cell lines with sera of PDAC patients. Here, we showed that HEK293T cells have undergone malignant transformation, increased the migration and invasion abilities, and acquired a partial chemoresistance, whereas hTERT-HPNE cells were almost refractory to transformation by patients' sera. Next-generation sequencing showed that transformed HEK293T cells gained and lost several genomic regions, harbouring genes involved in many cancer-associated processes. Our results support the genometastasis theory, but further studies are needed for the identification of the circulating transforming elements. Such elements could also be useful biomarkers in liquid biopsy assays.

Automated summary

This study suggests that the blood of PDAC patients may contain elements capable of transforming healthy cells by transferring oncogenes. The results support the genometastasis theory and suggest that these transforming elements could be useful biomarkers in liquid biopsy assays.