Influence of different buffers (HEPES/MOPS) on keratinocyte cell viability and microbial growth

This study assessed the effect of the buffers 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 3(N-morpholino) propanesulfonic acid (MOPS) on keratinocyte cell viability and microbial growth. It was observed that RPMI buffered with HEPES, supplemented with L-glutamine and sodium bicarbonate, can be used as a more suitable medium to promote co-culture. (C) 2016 Published by Elsevier B.V.