4.6 Article

A 3D printed mimetic composite for the treatment of growth plate injuries in a rabbit model

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NPJ REGENERATIVE MEDICINE
卷 7, 期 1, 页码 -

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NATURE PORTFOLIO
DOI: 10.1038/s41536-022-00256-1

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资金

  1. Eunice Kennedy Shriver National Institute of Child Health & Human Development of the National Institutes of Health (NIH) [R33HD090696]
  2. Gates Grubstake Fund
  3. graduate NIH Training Grant in Molecular Biophysics [T32 GM065103]
  4. Graduate Assistance in Areas of National Need (GAANN) from the Department of Education
  5. NSF Graduate Research Fellowship award
  6. GAANN award

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In this study, a 3D printed growth plate mimetic composite was developed to prevent limb length discrepancies and induce cartilage regeneration. The composite improved tibial lengthening in a rabbit model of growth plate injury, but did not show cartilage regeneration within the construct histologically.
Growth plate injuries affecting the pediatric population may cause unwanted bony repair tissue that leads to abnormal bone elongation. Clinical treatment involves bony bar resection and implantation of an interpositional material, but success is limited and the bony bar often reforms. No treatment attempts to regenerate the growth plate cartilage. Herein we develop a 3D printed growth plate mimetic composite as a potential regenerative medicine approach with the goal of preventing limb length discrepancies and inducing cartilage regeneration. A poly(ethylene glycol)-based resin was used with digital light processing to 3D print a mechanical support structure infilled with a soft cartilage-mimetic hydrogel containing chondrogenic cues. Our biomimetic composite has similar mechanical properties to native rabbit growth plate and induced chondrogenic differentiation of rabbit mesenchymal stromal cells in vitro. We evaluated its efficacy as a regenerative interpositional material applied after bony bar resection in a rabbit model of growth plate injury. Radiographic imaging was used to monitor limb length and tibial plateau angle, microcomputed tomography assessed bone morphology, and histology characterized the repair tissue that formed. Our 3D printed growth plate mimetic composite resulted in improved tibial lengthening compared to an untreated control, cartilage-mimetic hydrogel only condition, and a fat graft. However, in vivo the 3D printed growth plate mimetic composite did not show cartilage regeneration within the construct histologically. Nevertheless, this study demonstrates the feasibility of a 3D printed biomimetic composite to improve limb lengthening, a key functional outcome, supporting its further investigation as a treatment for growth plate injuries.

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