期刊
SMALL METHODS
卷 6, 期 11, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smtd.202200761
关键词
ionic amplification; nanofluidics; nanopores; permittivity gradients; translocation dynamics
资金
- Japan Society for the Promotion of Science (JSPS) KAKENHI [22H01926, 22K04893]
A permittivity gradient approach is reported to amplify the ionic blockade characteristics of DNA in solid-state nanopore sensing. Positive gradients enhance ionic signals, while negative gradients cause adverse effects. This phenomenon provides a novel way to enhance the single-molecule sensitivity of nanopore sensing.
Ionic signal amplification is a key challenge for single-molecule analyses by solid-state nanopore sensing. Here, a permittivity gradient approach for amplifying ionic blockade characteristics of DNA in a nanofluidic channel is reported. The transmembrane ionic current response is found to change substantially through modifying the liquid permittivity at one side of a pore with an organic solvent. Imposing positive liquid permittivity gradients with respect to the direction of DNA electrophoresis, this study observes the resistive ionic signals to become larger due to the varying contributions of molecular counterions. On the contrary, negative gradients render adverse effects causing conductive ionic current pulses upon polynucleotide translocations. Most importantly, both the positive and negative gradients are demonstrated to be capable of amplifying the ionic signals by an order of magnitude with a 1.3-fold difference in the transmembrane liquid dielectric constants. This phenomenon allows a novel way to enhance the single-molecule sensitivity of nanopore sensing that may be useful in analyzing secondary structures and genome sequence of DNA by ionic current measurements.
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