4.4 Article

Survey of porcine circovirus type 2 and parvovirus in swine breeding herds of Colombia

期刊

VETERINARY MEDICINE AND SCIENCE
卷 8, 期 6, 页码 2451-2459

出版社

WILEY
DOI: 10.1002/vms3.949

关键词

molecular characterization; reproductive failures in sows; PCVAD; PCV2d

资金

  1. Laboratory of Immunology and Molecular Biology - LIBM of the University of Tolima
  2. Corporacion Universitaria Remington

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In this study, the prevalence of porcine circovirus 2 (PCV2) and porcine parvovirus (PVP) in the department of Tolima, Colombia, was investigated. The results showed that PCV2d was detected in 90% of samples, while PVP was found in 2.6% of samples. Furthermore, genetic variations in the PCV2 capsid gene were identified, which may be associated with virus pathogenicity.
Background: One of the consequences of the presentation of reproductive failures in sows is the economic losses in production because it alters the estimated values of the volume of production, decreasing the productivity of the farm. Porcine circovirosis by porcine circovirus 2 (PCV2) has been associated with reproductive disorders, and porcine parvovirus (PVP) is one of the pathological agents most related to the presentation of reproductive failure in pigs. In Colombia, there are reports of the presence of PCV2 through molecular techniques, and PVP through serum tests; however, in the department of Tolima, the prevalence of these two viruses is unknown. Objective: In this study, the aim was to establish a report of the prevalence of viruses in five municipalities of the department of Tolima-Colombia. Methods: Blood samples from 150 breeding sows of five municipalities in Tolima, Colombia, were obtained. Quantitative polymerase chain reaction (q PCR) was used to detect the PCV2 and PVP virus in the blood samples followed by PCR and sequencing of 16 PCR products of the amplification of the cap gene of PCV2. A phylogenetic tree was constructed to identify the genotype of the PCV2 virus. Results: The presence of PCV2d in sows was detected in 135 samples (90%), as well as the identification of PVP in 2.6% of the samples. In addition, the phylogenetic analysis showed that 16 isolates were the PCV2d2 genotype. Conclusion: PCV2d and PVP were found to coinfect the females, and the identification of variability in regions in the predicted amino acid sequence of the PCV2 capsid may be associated with virus pathogenicity.

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