期刊
FRONTIERS IN VETERINARY SCIENCE
卷 9, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fvets.2022.954657
关键词
porcine rotavirus; VP6 gene; RT-RAA; RT-qPCR methods; detection assay
资金
- Science and Technology Plan Project of Sichuan Province
- [2020YFN0147]
In this study, a rapid and specific diagnostic method for porcine rotavirus type A (PoRVA) was successfully developed. The method utilized specific primers and probes designed based on the PoRVA VP6 gene, and obtained detection results that were completely consistent with the TaqMan probe-based RT-qPCR method in clinical samples.
Porcine rotavirus type A (PoRVA) is the main cause of dehydration and diarrhea in piglets, which has a great impact on the development of the pig industry worldwide. A rapid, accurate and sensitive detection method is conducive to the monitoring, control, and removal of PoRVA. In this study, a PoRVA real-time fluorescent reverse transcription recombinase-aided amplification (RT-RAA) assay was developed. Based on the PoRVA VP6 gene, specific primers and probes were designed and synthesized. The sensitivity of RT-RAA and TaqMan probe-based RT-qPCR was 7 copies per reaction and 5 copies per reaction, respectively. The sensitivity of the RT-RAA method was close to TaqMan probe-based RT-qPCR. The detection results of RT-RAA and TaqMan probe-based quantitative real-time RT-PCR methods were completely consistent in 241 clinical samples. Therefore, we successfully established a rapid and specific RT-RAA diagnostic method for PoRVA.
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