4.6 Article

Trace Analysis of Emerging Virus: An Ultrasensitive ECL-Scan Imaging System for Viral Infectious Disease

期刊

ACS OMEGA
卷 7, 期 42, 页码 37499-37508

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acsomega.2c04280

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资金

  1. National Key R&D Program of China [2021YFC2302200]
  2. Natural Science Fund of Guangdong Province for Distinguished Young Scholars [22050000282]
  3. National Natural Science Foundation of China [81972019, 21904145, 82102360, 81772136]
  4. Special Fund of Foshan Summit Plan [2020B019, 2020B012, 2020A015]
  5. Zhongnanshan Medical Foundation of Guangdong Province [ZNSA-2021012]
  6. Training project of National Science Foundation for Outstanding/Distinguished Young Scholars of Southern Medical University [C620PF0217]
  7. China Postdoctoral Science Foundation [2021M690634]
  8. Guangdong Basic and Applied Basic Research Foundation [2020A1515110529]
  9. Foundation of Foshan City [FS0AA-KJ218-1301-0034, 2018AB003411]
  10. Guangzhou Institute of Respiratory Health Open Project (China Evergrande Group) [2020GIRHHMS04]

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Emerging infectious diseases have significant impacts on human society. This study developed a dendritic polymer probe-amplified imaging system for trace analysis of viral emerging infectious diseases. The system showed high sensitivity and specificity in detecting Zika virus and SARS-CoV-2.
Emerging infectious diseases have brought a huge impact on human society in recent years. The outbreak of Zika virus (ZIKV) in the Americas resulted in a large number of babies born with microcephaly. More seriously, the Coronavirus Disease 2019 (COVID-19) was globally spread and caused immeasurable damages. Thus, the monitoring of highly pathogenic viruses is important to prevent and control emerging infectious diseases. Herein, a dendritic polymer probe-amplified ECL-scan imaging system was constructed to realize trace analysis of viral emerging infectious diseases. A dendritic polymer probe was employed as the efficient signal emitter component that could generate an amplified ECL signal on the integrated chip, and the signal was detected by a single-photon level charge coupled device-based ECL-scan imaging system. With this strategy, the ZIKV in a complex system of blood, urine, and saliva was detected. The results indicated that a high sensitivity of 50 copies and superior specificity were achieved. Furthermore, this strategy realized highly sensitive detection (10 copies) of the S and N protein gene sequence of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-Cov2) and spiked pseudovirus samples. Thus, the dendritic polymer probe-amplified ECL-scan imaging system suitably met the strict clinical requirements for trace analysis of an emerging virus, and thus has the potential to serve as a paradigm for monitoring emerging infectious diseases.

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