期刊
ACS OMEGA
卷 7, 期 34, 页码 30015-30027出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsomega.2c03023
关键词
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资金
- Cost Action [CA-17104]
This study prepared radiolabeled trastuzumab loaded solid lipid nanoparticles and investigated their biocompatibility, cellular uptake, and apoptosis induction capacity. The results showed that these nanoparticles exhibited favorable physicochemical properties and pharmacokinetic profiles.
Radiolabeled trastuzumab (TRZ) loaded solid lipid nano-particles (SLNs) were prepared by high shear homogenization and sonication techniques. The apoptosis mechanism of TRZ-SLNs was studied only with the MCF-7 cell line, while the cytotoxicity and cell binding capacity were investigated using breast cancer cells (MCF-7 and MDA-MB-231) and the human keratinocyte cell line (HaCaT). The particle sizes of TRZ-SLNs were found to be below 100 nm, and they possessed a negative charge. The high radiolabeling efficiency and good radiolabeling stability in saline and a cell culture medium were obtained in the results of radiolabeling studies. According to the in vitro studies, TRZ-SLNs were found to be biocompatible, and they effectively induced apoptosis in MCF-7 cells. After the parenteral injection of TRZ-SLNs into rats, a sustained release profile in blood circulation was achieved compared with free drug solution by the evaluation of pharmacokinetic parameters. As a conclusion, the study reveals that Technetium-99m (Tc-99m radiolabeled) TRZ loaded SLN formulations could be promising theranostic agents based on their characterization profiles, in vitro cellular uptake and apoptosis induction capacity, and in vivo pharmacokinetic profiles.
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