期刊
PLANTS-BASEL
卷 11, 期 17, 页码 -出版社
MDPI
DOI: 10.3390/plants11172244
关键词
Salvia; high throughput sequencing; repeatome; transposable elements; 45S rDNA; 5S rDNA; satellite DNA; FISH chromosome mapping
资金
- Russian Science Foundation [22-26-00222]
This study conducted a comparative analysis of the genome organization and chromosome structure of the important species Salvia officinalis and Salvia sclarea. The results revealed interspecific variations in repetitive DNA and identified potential chromosome markers. This research provides valuable insights into the taxonomy and genetic diversity of these two Salvia species.
Within the complicated and controversial taxonomy of cosmopolitan genus Salvia L. (Lamiaceae) are valuable species Salvia officinalis L. and Salvia sclarea L., which are important for the pharmaceutical, ornamental horticulture, food, and perfume industries. Genome organization and chromosome structure of these essential oil species remain insufficiently studied. For the first time, the comparative repeatome analysis of S. officinalis and S. sclarea was performed using the obtained NGS data, RepeatExplorer/TAREAN pipelines and FISH-based chromosome mapping of the revealed satellite DNA families (satDNAs). In repeatomes of these species, LTR retrotransposons made up the majority of their repetitive DNA. Interspecific variations in genome abundance of Class I and Class II transposable elements, ribosomal DNA, and satellite DNA were revealed. Four (S. sclarea) and twelve (S. officinalis) putative satDNAs were identified. Based on patterns of chromosomal distribution of 45S rDNA; 5S rDNA and the revealed satDNAs, karyograms of S. officinalis and S. sclarea were constructed. Promising satDNAs which can be further used as chromosome markers to assess inter- and intraspecific chromosome variability in Salvia karyotypes were determined. The specific localization of homologous satDNA and 45S rDNA on chromosomes of the studied Salvia species confirmed their common origin, which is consistent with previously reported molecular phylogenetic data.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据