期刊
PLANTS-BASEL
卷 11, 期 18, 页码 -出版社
MDPI
DOI: 10.3390/plants11182350
关键词
mulberry (Morus alba L.); drought stress; quantitative proteomics; antioxidant enzymes; glutathione peroxidase (GPX); reactive oxygen species (ROS)
资金
- Natural Science Basic Research Project in Shaanxi Province [2022JM-121, 2019JM-156]
- National Natural Science Foundation of China [32070197, 31570181]
- China Agriculture Research System [CARS-18]
This study elucidated the molecular mechanism of mulberry's response to drought stress using quantitative proteomics. The results showed that the GPX-based antioxidant enzymes play a crucial role in modulating mulberry's response to drought stress, and higher levels of GPX improve drought tolerance by enhancing the capacity of the antioxidant system for ROS scavenging.
Mulberry (Morus alba L.) has been an economically important food crop for the domesticated silkworm, Bombyx mori, in China for more than 5000 years. However, little is known about the mechanism underlying mulberry response to environmental stress. In this study, quantitative proteomics was applied to elucidate the molecular mechanism of drought response in mulberry. A total of 604 differentially expressed proteins (DEPs) were identified via LC-MS/MS. The proteomic profiles associated with antioxidant enzymes, especially five glutathione peroxidase (GPX) isoforms, as a scavenger of reactive oxygen species (ROS), were systematically increased in the drought-stressed mulberry. This was further confirmed by gene expression and enzymatic activity. Furthermore, overexpression of the GPX isoforms led to enhancements in both antioxidant system and ROS-scavenging capacity, and greater tolerance to drought stress in transgenic plants. Taken together, these results indicated that GPX-based antioxidant enzymes play an important role in modulating mulberry response to drought stress, and higher levels of GPX can improve drought tolerance through enhancing the capacity of the antioxidant system for ROS scavenging.
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