Evidence of Asexual Overwintering of Melampsora paradoxa and Mapping of Stem Rust Host Resistance in Salix

Melampsora rust is a devastating disease of shrub willow in North America. Previous work has identified Melampsora paradoxa as one of two identified rust species in New York State that infect Salix purpurea and other important Salix host species, however little is known about the population of this rust species in this region. Genotyping-by-sequencing was used to identify single nucleotide polymorphisms (SNPs) and assess population diversity of M. paradoxa isolates collected from three Salix breeding populations in Geneva, NY between 2015 and 2020. Statistical analyses of SNP revealed that all isolates collected were clonally derived even though they were collected across years. In 2020, isolates were collected from stem infections where uredospore pustules were observed, and these isolates were also identical to M. paradoxa collected in previous seasons. These data suggest that M. paradoxa sampled across multiple years overwintered and reproduced asexually and that stem infection is a possible mechanism for overwintering, both of which are novel findings for this rust species. Additionally, field disease ratings were conducted on a S. purpurea x S. suchowensis F-1 breeding population with high disease severity, enabling the discovery of QTL for resistance on chromosomes 1 and 19. Lastly, Colletotrichum salicis was frequently associated with stem rust and may play a role in M. paradoxa stem infection. Together, this work is the first substantial exploration into M. paradoxa population biology, stem infection, and host resistance in Salix.

Automated summary

This study provides the first substantial exploration into the population biology, stem infection, and host resistance of Melampsora paradoxa in Salix. The research revealed that the isolates collected from different years in a specific region of New York State were clonally derived, indicating a high degree of population uniformity. It was also found that M. paradoxa may overwinter and reproduce asexually through stem infection, which is a novel finding for this rust species. Additionally, QTL for resistance were discovered on chromosomes 1 and 19 in a S. purpurea x S. suchowensis F-1 breeding population with high disease severity. Furthermore, the association of Colletotrichum salicis with stem rust suggests its potential role in M. paradoxa stem infection.