期刊
JOURNAL OF MEMBRANE BIOLOGY
卷 249, 期 4, 页码 475-481出版社
SPRINGER
DOI: 10.1007/s00232-016-9888-z
关键词
Trypanosoma cruzi; Polyamine transporter; Chagas disease
资金
- Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET) [PIP 2011-0263, 2013-0664]
- Agencia Nacional de Promocion Cientifica y Tecnologica (FONCYT PICT) [2012-0559, 2013-2218]
Trypanosoma cruzi is the etiological agent of Chagas disease, a major health problem in Latin America. Polyamines are polycationic compounds that play a critical role as regulators of cell growth and differentiation. In contrast with other protozoa, T. cruzi is auxotrophic for polyamines because of its inability to synthesize putrescine due to the lack of both, arginine and ornithine decarboxylase; therefore, the intracellular availability of polyamines depends exclusively on transport processes. In this work, the polyamine transporter TcPAT12 was overexpressed in T. cruzi epimastigotes demonstrating that growth rates at different concentrations of polyamines strongly depend on the regulation of the polyamine transport. In addition, parasites overexpressing TcPAT12 showed a highly increased resistance to hydrogen peroxide and the trypanocidal drugs nifurtimox and benznidazole, which act by oxidative stress and interfering the synthesis of polyamine derivatives, respectively. Finally, the presence of putative polyamine transporters was analyzed in T. cruzi, Trypanosoma brucei, and Leishmania major genomes identifying 3-6 genes in these trypanosomatids.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据