4.6 Article

Ribes nigrum L. Extract-Mediated Green Synthesis and Antibacterial Action Mechanisms of Silver Nanoparticles

期刊

ANTIBIOTICS-BASEL
卷 11, 期 10, 页码 -

出版社

MDPI
DOI: 10.3390/antibiotics11101415

关键词

silver nanoparticles; Ribes nigrum; natural products; phytochemical investigation; antimicrobial

资金

  1. Science Committee of RA [21AG-4D027]
  2. Science Committee of RA, Ministry of Education, Science, Culture and Sports of RA
  3. EU Erasmus + Exchange Program by Saarland University, Germany

向作者/读者索取更多资源

Silver nanoparticles (Ag NPs) were synthesized using black currant leaf extract and evaluated for their antibacterial activities. The extract itself had no antibacterial effect, but the Ag NPs showed significant activity against bacteria. Further experiments revealed that the biogenic Ag NPs affected the membrane-associated properties of bacteria.
Silver nanoparticles (Ag NPs) represent one of the most widely employed metal-based engineered nanomaterials with a broad range of applications in different areas of science. Plant extracts (PEs) serve as green reducing and coating agents and can be exploited for the generation of Ag NPs. In this study, the phytochemical composition of ethanolic extract of black currant (Ribes nigrum) leaves was determined. The main components of extract include quercetin rutinoside, quercetin hexoside, quercetin glucuronide, quercetin malonylglucoside and quercitrin. The extract was subsequently employed for the green synthesis of Ag NPs. Consequently, R. nigrum leaf extract and Ag NPs were evaluated for potential antibacterial activities against Gram-negative bacteria (Escherichia coli ATCC 25922 and kanamycin-resistant E. coli pARG-25 strains). Intriguingly, the plant extract did not show any antibacterial effect, whilst Ag NPs demonstrated significant activity against tested bacteria. Biogenic Ag NPs affect the ATPase activity and energy-dependent H+-fluxes in both strains of E. coli, even in the presence of N,N'-dicyclohexylcarbodiimide (DCCD). Thus, the antibacterial activity of the investigated Ag NPs can be explained by their impact on the membrane-associated properties of bacteria.

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