Transcriptomic and Metabolomic Analyses Providing Insights into the Coloring Mechanism of Docynia delavayi

The metabolome and transcriptome profiles of three different variations of mature Docynia delavayi fruit were synthesized to reveal their fruit color formation mechanism. A total of 787 secondary metabolites containing 149 flavonoid metabolites, most of which were flavonoids and flavonols, were identified in the three variations using ultra performance liquid chromatography- tandem mass spectrometry (UPLC-MS/MS), and we found that the secondary metabolites cyanidin-3-O-galactoside and cyanidin-3-O-glucoside were the major coloring substances in D. delavayi. This was associated with the significant upregulation of the structural genes F3H and F3'H in the anthocyanin synthesis pathway and the control genes WRKY, MYB, bZIP, bHLH, and NAC in RP. F3'H expression may play a significant role in the selection of components for anthocyanin synthesis. Our results contribute to breeding and nutritional research in D. delavayi and provide insight into metabolite studies of the anthocyanin biosynthetic pathway.

Automated summary

The metabolome and transcriptome profiles of different variations of Docynia delavayi fruit were analyzed to understand the mechanism of fruit color formation. Flavonoid metabolites were identified as the major coloring substances, and the expression of certain genes in the anthocyanin synthesis pathway was found to be significantly upregulated. This study provides important insights for breeding and nutritional research in D. delavayi.