期刊
MICROORGANISMS
卷 10, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms10101888
关键词
proline; Staphylococcus aureus; transport; bacterial pathogenesis
类别
资金
- NIH [AI47801]
Staphylococcus aureus requires the importation of proline to survive. Single mutations in proline transporter genes affect the ability of the bacterium to survive, while triple gene mutations significantly hinder the proline transport and growth of S. aureus.
Staphylococcus aureus is a major cause of skin/soft tissue infections and more serious infections in humans. The species usually requires the importation of proline to be able to survive. Previous work has shown that single mutations in genes that encode for proline transporters affect the ability of S. aureus to survive in vitro and in vivo. To better understand proline transport in S. aureus, double and triple gene mutant strains were created that targeted the opuD, proP, and putP genes. Single gene mutants had some effect on proline transport, whereas double mutants exhibited significantly lower proline transport. An opuD prop putP triple gene mutant displayed the lowest proline transport under low- and high-affinity conditions. To assess growth differences caused by the mutations, the same mutants were grown in brain heart infusion (BHI) broth and defined staphylococcal medium (DSM) with various concentrations of proline. The triple mutant did not grow in DSM with a low concentration of proline and grew poorly in both DSM with a high proline concentration and BHI broth. These results show that S. aureus has multiple mechanisms to import proline into the cell and knocking out three of the main proline transporters significantly hinders S. aureus growth.
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