Improvement of Freeze-Dried Survival of Lactiplantibacillus plantarum Based on Cell Membrane Regulation

The cell membrane of Lactiplantibacillus plantarum is a key structure for cell survival. In this study, we aimed to improve the lyophilization resistance of L. plantarum by regulating the cell membrane structure. Unsaturated fatty acids or cell membrane-regulating substances were added during culturing to determine their effect on the composition of cell membrane fatty acids and the survival rate of the cells after freeze-drying. The results showed that Tween 80, beta-carotene and melatonin increased the lyophilization survival rate of L. plantarum by 9.44, 14.53, and 18.34%, respectively. After adding a lyophilization protective agent at a concentration of 21.49% at a 1:1 ratio, a combination of Tween 80, melatonin, and beta-carotene was added to regulate the cell membrane, which increased the lyophilization survival rate by 32.08-86.05%. This study proposes new research directions and ideas for improving the survival rate of probiotics for industrial production.

Automated summary

The aim of this study was to improve the lyophilization resistance of L. plantarum by regulating the cell membrane structure. The results showed that the addition of Tween 80, beta-carotene, and melatonin significantly increased the lyophilization survival rate of L. plantarum. This study provides new directions for improving the industrial production of probiotics.