期刊
ANTIOXIDANTS
卷 11, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/antiox11101897
关键词
NRF2; amyotrophic lateral sclerosis; C9orf72; dipeptide repeat proteins
资金
- Spanish Ministry of Economy and Competitiveness [SAF2019-110061RB-100]
- Spanish Foundation - Network of Centers of Excellence in Neurodegeneration [P-024-FTPGB 2018]
- UK Medical Research Council
- Spanish Instituto de Salud Carlos III [PI19/01030]
- Spanish Ministry of Universities [FPU20/03326]
- Autonomous University of Madrid
- Motor Neurone Disease Association [AMBRoSIA 972-797, NECTAR 974-797]
- NIHR Sheffield Biomedical Research Centre [JS-BRC-1215-20017]
- COST (European Cooperation in Science and Technology) [CA20121]
The hexanucleotide expansion of the C9orf72 gene is associated with familial amyotrophic lateral sclerosis (ALS). This study found that the expression of redox-related genes was altered in leukocytes from C9orf72 ALS patients. Further experiments revealed that exposure to dipeptide repeat (DPR) toxicity decreased NRF2 protein levels, leading to increased superoxide levels and reduced cell survival. However, activation of NRF2 was able to restore cell viability and redox homeostasis. This suggests that NRF2 may be a potential target for precision medicine in treating ALS patients with C9orf72 expansion repeats.
The hexanucleotide expansion of the C9orf72 gene is found in 40% of familial amyotrophic lateral sclerosis (ALS) patients. This genetic alteration has been connected with impaired management of reactive oxygen species. In this study, we conducted targeted transcriptional profiling in leukocytes from C9orf72 patients and control subjects by examining the mRNA levels of 84 redox-related genes. The expression of ten redox genes was altered in samples from C9orf72 ALS patients compared to healthy controls. Considering that Nuclear factor erythroid 2-Related Factor 2 (NRF2) modulates the expression of a wide range of redox genes, we further investigated its status on an in vitro model of dipeptide repeat (DPR) toxicity. This model mimics the gain of function, toxic mechanisms attributed to C9orf72 pathology. We found that exposure to DPRs increased superoxide levels and reduced mitochondrial potential as well as cell survival. Importantly, cells overexpressing DPRs exhibited reduced protein levels of NRF2 and its target genes upon inhibition of the proteasome or its canonical repressor, the E3 ligase adapter KEAP1. However, NRF2 activation was sufficient to recover cell viability and redox homeostasis. This study identifies NRF2 as a putative target in precision medicine for the therapy of ALS patients harboring C9orf72 expansion repeats.
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