期刊
ANTIOXIDANTS
卷 11, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/antiox11102055
关键词
metabolomics; Nrf2; ARE; oxidative stress; wine polyphenols
资金
- University of Verona, Italy, School of Medicine
This study investigates the antioxidant effects of polyphenols in red wine by examining their ability to pass through cell membranes and regulate gene expression. The results show that red wine polyphenols, specifically stilbenes, flavan-3-ols derivatives, and flavonoids, can enter cells and lower reactive oxygen species induced by tert-butyl hydroperoxide. Additionally, red wine extract increases the expression of Nrf2, hemeoxygenase-1, and glutamate-cysteine ligase catalytic subunit genes in the cells.
Moderate wine consumption has been associated with several benefits to human health due to its high polyphenol content. In this study, we investigated whether polyphenols contained in a particular red wine, rich in polyphenols, can pass the cell membrane and switch the oxidant/antioxidant balance toward an antioxidant pattern of THP-1 cells and human cardiomyocytes through a gene regulatory system. First, we identified which metabolite polyphenols present in red wine extract cross cell membranes and may be responsible for antioxidant effects. The results showed that the wine metabolites in treated cells belonged mainly to stilbenes, flavan-3-ols derivatives, and flavonoids. Other metabolites present in cells were not typical wine metabolites. Then, we found that red wine extract dose-dependently lowered reactive oxygen species (ROS) induced by tert-butyl hydroperoxide (TBHP) up to 50 +/- 7% in both cell lines (p < 0.01). Furthermore, wine extract increased nuclear Nrf2 of about 35 +/- 5% in both cell lines (p < 0.01) and counteracted its reduction induced by TBHP (p < 0.01). The rise in Nrf2 was paralleled by the increase in hemeoxygenase-1 and glutamate-cysteine ligase catalytic subunit gene expression (both mRNA and protein) (p < 0.01). These results could help explain the healthful activity of wine polyphenols within cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据