The Novel Potentiometric Approach to Antioxidant Capacity Assay Based on the Reaction with Stable Radical 2,2′-diphenyl-1-picrylhydrazyl

For the first time, new possibilities of using the DPPH center dot as a signal-forming oxidant molecule with potentiometric detection are shown. The CV method confirmed the presence of a quasi-reversible potential-determining system DPPH center dot/DPPH-H under experimental conditions. This fact makes it possible to use DPPH center dot as the model of the oxidizing agent for obtaining an analytical signal by the potentiometry method. The potentiometric approach makes it possible to obtain the value of the Nernst slope and the antioxidant capacity in one experiment. It consists of an antioxidant supplement and two consecutive DPPH center dot supplements. In this case, the calculation of the Nernst slope is carried out by introducing the second addition of the oxidizing agent and constructing a calibration curve against the reaction background with an antioxidant. Solutions of individual antioxidants alpha-tocopherol, quercetin, (+/-)-catechin hydrate, and alpha-lipoic acid were studied by the developed approach. A high correlation with the results of spectrophotometric measurements is shown. At the same time, the potentiometry method is devoid of the concentration limitations of the spectrophotometric method, which was confirmed. In the study of plant materials extracts, a high correlation of antioxidant capacity, obtained by potentiometric and spectrophotometric methods, was shown only for objects whose color did not contribute to the DPPH center dot absorption. The versatility of the potentiometric method for studying objects of any color was shown.

Automated summary

For the first time, the use of DPPH center dot as an oxidant molecule and its detection through potentiometry have been shown. The potentiometric method allows for the determination of Nernst slope and antioxidant capacity in one experiment, making it a versatile approach for studying materials of any color.