期刊
ANTIOXIDANTS
卷 11, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/antiox11091781
关键词
Lonicerae japonicae flos; human neutrophils; superoxide anion (O-2 (-)); calcium mobilization; SARS-CoV-2-Spike; ACE2; bioassay-coupled chromatographic profile
资金
- Chang Gung Memorial Hospital [CMRPG3J0231~3, CMRPF1J0051~3, BMRP450]
- Chang Gung University of Science and Technology [ZRRPF3K0111]
- Ministry of Education [DP2-111-21121-01-N-01-03]
- National Science and Technology Council [MOST 106-2320-B-255-003-MY3, MOST 108-2320-B-255-003-MY3, MOST 109-2327-B-255-001, MOST 111-2321-B-255-001, MOST 110-2320-B-038-013, MOST 110-2320-B-038-034, MOST 111-2320-B-038-040-MY3]
- Taipei Medical University, Taiwan [TMU109-AE1-B15]
The study found that the ethanol extract of Lonicerae japonicae flos has anti-inflammatory effects on human neutrophils activated by fMLF, suggesting its potential application in daily health care during the COVID-19 pandemic.
Lonicerae japonicae flos (LJ) is an Asian traditional herb that is used as a dietary supplement, tea, and beverage to clear heat and quench thirst. However, no studies investigated its effect on activated human neutrophils, which played a crucial role in the bad prognosis of coronavirus disease of 2019 (COVID-19) patients by aggravating lung inflammation and respiratory failure. Herein, we evaluated the anti-inflammatory effect of LJ ethanol extract (LJEE) on human neutrophils activated by N-formyl-methionyl-leucyl-phenylalanine (fMLF). Our experimental results indicated that LJEE suppressed fMLF-activated superoxide anion (O-2(center dot-)) generation, the expression of CD11b, and cell adhesion and migration, as well as the formation of neutrophil extracellular traps in human neutrophils. Further in-depth mechanical investigation revealed that pretreatment with LJEE accelerated the Ca2+ clearance, but did not affect the phosphorylation of mitogen-activated protein kinases (MAPKs) and protein kinase B (Akt) in activated human neutrophils. In addition, LJEE displayed a dose-dependent reactive oxygen species (ROS) scavenger activity, which assisted its anti-inflammatory activity. From the bioassay-coupled chromatographic profile, chlorogenic acids were found to dominate the anti-inflammatory effects of LJEE. Moreover, LJ water extract (LJWE) demonstrated an interrupting effect on the severe acute respiratory syndrome coronavirus-2 spike protein (SARS-CoV-2-Spike)/angiotensin-converting enzyme 2 (ACE2) binding. In conclusion, the obtained results not only supported the traditional use of LJ for heat-clearance, but also suggested its potential application in daily health care during the COVID-19 pandemic.
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