4.7 Article

Improving Precise Genome Editing Using Donor DNA/gRNA Hybrid Duplex Generated by Complementary Bases

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Biochemistry & Molecular Biology

A Cas9-transcription factor fusion protein enhances homology-directed repair efficiency

Guoling Li et al.

Summary: This study presents a strategy to enhance HDR efficiency in animal cells by spatial and temporal colocalization of the donor and Cas9 through coupling the CRISPR system with the transcription factor THAP11. By appending THAP11-specific DNA binding motifs to the double-stranded donor DNA, the HDR efficiency can be significantly improved. The THAP11-fused CRISPR system achieved a more than twofold increase in HDR-mediated knock-in efficiency, and up to 6-fold increase can be achieved through combinational use with the small-molecule HDR enhancer valnemulin.

JOURNAL OF BIOLOGICAL CHEMISTRY (2021)

Review Oncology

CRISPR Gene Therapy: Applications, Limitations, and Implications for the Future

Fathema Uddin et al.

FRONTIERS IN ONCOLOGY (2020)

Article Biotechnology & Applied Microbiology

Low-gluten, nontransgenic wheat engineered with CRISPR/Cas9

Susana Sanchez-Leon et al.

PLANT BIOTECHNOLOGY JOURNAL (2018)

Article Biotechnology & Applied Microbiology

Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos

Bin Gu et al.

NATURE BIOTECHNOLOGY (2018)

Review Multidisciplinary Sciences

The CRISPR tool kit for genome editing and beyond

Mazhar Adli

NATURE COMMUNICATIONS (2018)

Review Chemistry, Multidisciplinary

Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts

Martin P. Stewart et al.

CHEMICAL REVIEWS (2018)

Article Biochemical Research Methods

Genome editing via delivery of Cas9 ribonucleoprotein

Mark A. DeWitt et al.

METHODS (2017)

Article Multidisciplinary Sciences

Assembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing

Jared Carlson-Stevermer et al.

NATURE COMMUNICATIONS (2017)

Article Multidisciplinary Sciences

Fusion guide RNAs for orthogonal gene manipulation with Cas9 and Cpf1

Jiyeon Kweon et al.

NATURE COMMUNICATIONS (2017)

Article Multidisciplinary Sciences

Direct protein introduction into plant cells using a multi-gas plasma jet

Yuki Yanagawa et al.

PLOS ONE (2017)

Article Multidisciplinary Sciences

Precise genome-wide base editing by the CRISPR Nickase system in yeast

Atsushi Satomura et al.

SCIENTIFIC REPORTS (2017)

Review Biotechnology & Applied Microbiology

CRISPR/Cas9 advances engineering of microbial cell factories

Tadas Jakociunas et al.

METABOLIC ENGINEERING (2016)

Article Biotechnology & Applied Microbiology

CRISPR Repair Reveals Causative Mutation in a Preclinical Model of Retinitis Pigmentosa

Wen-Hsuan Wu et al.

MOLECULAR THERAPY (2016)

Article Cell Biology

Selection-free genome editing of the sickle mutation in human adult hematopoietic stem/progenitor cells

Mark A. DeWitt et al.

SCIENCE TRANSLATIONAL MEDICINE (2016)

Article Multidisciplinary Sciences

ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes

Kazuto Yoshimi et al.

NATURE COMMUNICATIONS (2016)

Article Biotechnology & Applied Microbiology

Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo

John A. Zuris et al.

NATURE BIOTECHNOLOGY (2015)

Article Biochemistry & Molecular Biology

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

James E. DiCarlo et al.

NUCLEIC ACIDS RESEARCH (2013)

Article Multidisciplinary Sciences

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

Martin Jinek et al.

SCIENCE (2012)

Article Biochemistry & Molecular Biology

Factors determining DNA double-strand break repair pathway choice in G2 phase

Atsushi Shibata et al.

EMBO JOURNAL (2011)

Review Genetics & Heredity

Regulation of Homologous Recombination in Eukaryotes

Wolf-Dietrich Heyer et al.

ANNUAL REVIEW OF GENETICS, VOL 44 (2010)