期刊
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
卷 10, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2022.959441
关键词
synthetic biology; bacteria; gut; microbiota; diagnostic; therapy; spatial; biogeography
资金
- Wellcome Trust
- Royal Society [211230/Z/18/Z]
- Imperial College London President's PhD Scholarship
- Wellcome Trust [211230/Z/18/Z] Funding Source: Wellcome Trust
The mammalian gut and its microbiome form a complex and dynamic environment, and engineered bacteria with synthetic genetic circuits can provide spatially precise diagnostics and therapeutics for specific biomarkers.
The mammalian gut and its microbiome form a temporally dynamic and spatially heterogeneous environment. The inaccessibility of the gut and the spatially restricted nature of many gut diseases translate into difficulties in diagnosis and therapy for which novel tools are needed. Engineered bacterial whole-cell biosensors and therapeutics have shown early promise at addressing these challenges. Natural and engineered sensing systems can be repurposed in synthetic genetic circuits to detect spatially specific biomarkers during health and disease. Heat, light, and magnetic signals can also activate gene circuit function with externally directed spatial precision. The resulting engineered bacteria can report on conditions in situ within the complex gut environment or produce biotherapeutics that specifically target host or microbiome activity. Here, we review the current approaches to engineering spatial precision for in vivo bacterial diagnostics and therapeutics using synthetic circuits, and the challenges and opportunities this technology presents.
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