4.7 Article

Light-mediated control of gene expression in the anoxygenic phototrophic bacterium Rhodobacter capsulatus using photocaged inducers

出版社

FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2022.902059

关键词

caged compounds; light-controlled gene expression; optogenetics; purple non-sulfur photosynthetic bacteria; Rhodobacter capsulatus

资金

  1. German Federal Ministry of Education and Research (BMBF)
  2. Bioeconomy Science Center
  3. project CLIB-Kompetenzzentrum Biotechnologie (CKB)
  4. European Regional Development Fund (ERDF)
  5. CLIB-Kompetenzzentrum Biotechnologie (CKB)
  6. Ministry of Culture and Science of the German federal state of North Rhine-Westphalia
  7. NRW Strategieprojekt BioSC
  8. [313/323-400-00213]
  9. [FKZ: 031A167A), 2]
  10. [34. EFRE-0300096]
  11. [34. EFRE-0300097]

向作者/读者索取更多资源

In this study, we successfully implemented a light-mediated on-switch for target gene expression in the facultative anoxygenic phototroph Rhodobacter capsulatus using photocaged IPTG. We also demonstrated its application in inducing intrinsic carotenoid biosynthesis.
Photocaged inducer molecules, especially photocaged isopropyl-beta-d-1-thiogalactopyranoside (cIPTG), are well-established optochemical tools for light-regulated gene expression and have been intensively applied in Escherichia coli and other bacteria including Corynebacterium glutamicum, Pseudomonas putida or Bacillus subtilis. In this study, we aimed to implement a light-mediated on-switch for target gene expression in the facultative anoxygenic phototroph Rhodobacter capsulatus by using different cIPTG variants under both phototrophic and non-phototrophic cultivation conditions. We could demonstrate that especially 6-nitropiperonyl-(NP)-cIPTG can be applied for light-mediated induction of target gene expression in this facultative phototrophic bacterium. Furthermore, we successfully applied the optochemical approach to induce the intrinsic carotenoid biosynthesis to showcase engineering of a cellular function. Photocaged IPTG thus represents a light-responsive tool, which offers various promising properties suitable for future applications in biology and biotechnology including automated multi-factorial control of cellular functions as well as optimization of production processes.

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