Kidney collecting duct cells make vasopressin in response to NaCl-induced hypertonicity

Vasopressin has traditionally been thought to be produced by the neurohypophyseal system and then released into the circulation where it regulates water homeostasis. The questions of whether vasopressin could be produced outside of the brain and if the kidney could be a source of vasopressin are raised by the syndrome of inappropriate antidiuretic hormone secretion (vasopressin). We found that mouse and human kidneys expressed vasopressin mRNA. Using an antibody that detects preprovasopressin, we found that immunoreactive preprovasopressin protein was found in mouse and human kidneys. Moreover, we found that murine collecting duct cells made biologically active vasopressin, which increased in response to NaCl-mediated hypertonicity, and that water restriction increased the abundance of kidney-derived vasopressin mRNA and protein expression in mouse kidneys. Thus, we provide evidence of biologically active production of kidney -derived vasopressin in kidney tubular epithelial cells.

Automated summary

Research has found that vasopressin can be produced in the kidneys, specifically in the tubular epithelial cells of mouse and human kidneys. This kidney-derived vasopressin is biologically active and regulated by hypertonic conditions and water restriction.