期刊
JOURNAL OF EXTRACELLULAR VESICLES
卷 11, 期 9, 页码 -出版社
WILEY
DOI: 10.1002/jev2.12254
关键词
neuroglia; exosomes; tetraspanin; gliosis; SNARE complex; secretome; vitreous body; microvillus
类别
资金
- Universite de Strasbourg [UPR3212]
- ProRetina Foundation Germany [Pro-Re/Seed/Grosche.1-2014, Pro-Re/Seed/Kaplan-Grosche.1-2019]
- Centre National de la Recherche Scientifique [UPR3212]
- Deutsche Forschungsgemeinschaft [GR 4403/1-1, GR 4403/5-1, GR 4403/7-1, HA 6014/5-1]
- Agence Nationale de la Recherche
This study reveals the communication mechanism of Muller cells in the retina of adult mice through the release of extracellular vesicles. The Muller cell-specific SNARE protein VAMP5 is identified as a component of extracellular vesicles and is responsive to ischemia. Furthermore, differences in the secretomes between Muller cells and neurons in vitro are uncovered.
Cell-cell interactions in the central nervous system are based on the release of molecules mediating signal exchange and providing structural and trophic support through vesicular exocytosis and the formation of extracellular vesicles. The specific mechanisms employed by each cell type in the brain are incompletely understood. Here, we explored the means of communication used by Muller cells, a type of radial glial cells in the retina, which forms part of the central nervous system. Using immunohistochemical, electron microscopic, and molecular analyses, we provide evidence for the release of distinct extracellular vesicles from endfeet and microvilli of retinal Muller cells in adult mice in vivo. We identify VAMP5 as a Muller cell-specific SNARE component that is part of extracellular vesicles and responsive to ischemia, and we reveal differences between the secretomes of immunoaffinity-purified Muller cells and neurons in vitro. Our findings suggest extracellular vesicle-based communication as an important mediator of cellular interactions in the retina.
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