4.5 Article

Enzymatic bioconversion of beechwood xylan into the antioxidant 2′-O-α-(4-O-methyl-D-glucuronosyl)-xylobiose

期刊

出版社

SPRINGER HEIDELBERG
DOI: 10.1007/s13399-022-03240-3

关键词

Hemicellulose; Endoxylanase; Bioactive oligosaccharides; Xylooligosaccharides; Antioxidants; Aldouronic acids; Acidic xylooligosaccharides; Prebiotics

资金

  1. CRUE-CSIC
  2. Springer Nature
  3. Spanish Ministry of Economy and Competitiveness [PID2019-105838RB-C31]
  4. Spanish Ministry of Education [FPU18/01866]

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This study presents a simple method for the production of acidic xylooligosaccharides (XOS) from hardwood hemicellulose through enzymatic hydrolysis. The enzyme Depol 670L displayed the highest activity and resulted in high yield of acidic XOS. The acidic XOS exhibited high antioxidant activity.
Acidic xylooligosaccharides (XOS), also called aldouronics, are hetero-oligomers of xylose randomly branched with 4-O-methyl-D-glucuronic acid residues linked by alpha(1 -> 2) bonds, which display bioactive properties. We have developed a simple and integrated method for the production of acidic XOS by enzymatic hydrolysis of a glucurono-xylan from beechwood. Among the enzymes screened, Depol 670L (a cellulolytic preparation from Trichoderma reesei) displayed the highest activity (70.3 U/mL, expressed in reducing xylose equivalents). High-performance anion-exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD) analysis revealed the formation of a neutral fraction (corresponding to linear XOS, mainly xylose and xylobiose) and a group of more retained products (acidic XOS), which were separated using strong anion-exchange cartridges. The acidic fraction contained a major product, characterized by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry and mono- and two-dimensional nuclear magnetic resonance spectroscopy (NMR) as 2 '-O-alpha-(4-O-methyl-alpha-D-glucuronosyl)-xylobiose (X2_MeGlcA). Starting from 2 g of beechwood xylan, 1.5 g of total XOS were obtained, from which 225 mg (11% yield) corresponded to the aldouronic X2_MeGlcA. The acidic XOS exhibited higher antioxidant activity (measured by the ABTS(center dot+) discoloration assay) than xylan, whilst neutral XOS displayed no antioxidant activity. This work demonstrates that it is possible to obtain a safe and natural antioxidant by enzymatic biotransformation of hardwood hemicellulose.

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