Enhancement of the Expression of ZmBZR1 and ZmBES1 Regulatory Genes and Antioxidant Defense Genes Triggers Water Stress Mitigation in Maize (Zea mays L.) Plants Treated with 24-Epibrassinolide in Combination with Spermine

Water shortages greatly threaten global food security and limit crop production. Hence, increasing crop water stress tolerance is a critical way to secure agricultural production. 24-Epibrassinolide (EBL) and spermine (Spm) are closely involved in plant growth and development, as well as stress tolerance. In this study, the potential role of 0.1 mg L-1 EBL and/or 25 mg L-1 Spm foliage applications in improving the tolerance of maize to water-deficit conditions (50% and 75% field capacity) was investigated. We found that EBL, either alone or in combination with Spm, plays a major role in maize drought tolerance through upregulating the expression of both regulatory genes (ZmBZR1 and ZmBES1) of the brassinosteroid signal transduction pathway and gene-encoding antioxidant defense enzymes ZmSOD, ZmCAT, ZmAPX, ZmMDHAR, ZmDHAR, and ZmGR. Moreover, exogenous treatments alleviated the inhibition of maize plant growth and productivity and mitigated drought-induced oxidative stress by improving antioxidant enzyme (superoxide dismutase, catalase, ascorbate peroxidase, dehydroascorbate reductase, monodehydroascorbate reductase, glutathione reductase) activity, enhancing antioxidant molecule (ascorbate, glutathione) content, preventing reactive oxygen species accumulation, and maintaining cell membrane integrity. These findings reveal that the application of EBL, either individually or in combination with Spm, can be a good strategy for ameliorating water stress in sustainable agricultural systems.

Automated summary

Water shortages pose a significant threat to global food security and crop production, making it crucial to enhance crop tolerance to water stress. This study investigates the potential role of 0.1 mg L-1 24-epibrassinolide (EBL) and/or 25 mg L-1 spermine (Spm) in improving maize's tolerance to water-deficit conditions. The results show that EBL, alone or in combination with Spm, plays a major role in enhancing maize drought tolerance by regulating gene expression and increasing antioxidant defense enzymes. Furthermore, these treatments alleviate growth inhibition and oxidative stress, thus mitigating the negative effects of drought. Overall, this research highlights the significance of using EBL, either individually or in combination with Spm, to alleviate water stress in agriculture.