期刊
AGRONOMY-BASEL
卷 12, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/agronomy12112592
关键词
oil tea; ploidy; pollen size; pollen viability; pollen germination rate
资金
- Special Funds for Construction of Innovative Provinces in Hunan Province [2021NK1007]
- National Natural Science Foundation of China [31500553]
- National Key R&D Program of China [2018YFD1000603]
Oil tea (Camellia oleifera Abel.) is an important woody edible oil tree in China with intraspecific polyploid. The study found that pollen size is positively correlated with ploidy level in oil tea, and the hexaploid had relatively high pollen viability. This knowledge provides basic information for breeding plans and pollination methods of oil tea.
Oil tea (Camellia oleifera Abel.) is one of the most important woody edible oil tree species in China, with intraspecific polyploid. In order to study the variation in pollen size and vigor of C. oleifera at ploidy level, four ploidy covers a total of 32 types of Camellia pollens as the material for the experiment. The results showed that the pollen sizes of diploid, tetraploid, hexaploidy, and octaploid were positively correlated with the ploidy level. Pollen viability of C. oleifera was determined by fluorescein diacetate (FDA) dye solution staining and medium containing 10% sucrose, 0.01% boric acid, and 1% agar germination in vitro, which indicated that the pollen viability and germination rate of the hexaploid were relatively high among the four ploidy levels, at 79.69% and 71.78% respectively. The pollen vigor of diploid NR-3, tetraploid DP43, hexaploid CJ-12, and octoploid YNYC-1 was higher than that of other materials with the same ploidy level. Knowledge of different ploidy pollen sizes and pollen viability provides basic information for formulating pollen breeding plans and pollination methods of C. oleifera.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据