期刊
AGRONOMY-BASEL
卷 12, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/agronomy12102573
关键词
non-heading Chinese cabbage; BcHY5; photomorphogenesis; hypocotyl; BcBBX24
资金
- China Agriculture Research System [CARS-23-A-16]
- Key Projects of the National Key Research and Development Plan [2017YFD0101803]
- Nanjing Science and Technology project [202109022]
In this study, we identified a crucial transcription factor BcHY5 and its regulator BcBBX24, which play important roles in photomorphogenesis of hypocotyl in non-heading Chinese cabbage seedlings.
The primary domain/leucine zipper (bZIP) transcription factor, Elongated Hypocotyl (HY5), is crucial for the photomorphogenesis of seedlings. Here, we identified BcHY5 as a regulator of hypocotyl length from the non-heading Chinese cabbage (NHCC) variety 'Suzhouqing', which encoded a protein comprised 164 amino acid residues. Ectopic expression of BcHY5 in Arabidopsis shortens the length of the hypocotyl. Additionally, we discovered a protein called BcBBX24 containing the B-BOX (BBX) domain, which is the interacting partner of BcHY5. Yeast two-hybrid (Y2H), bimolecular fluorescence complementation (BiFC) and GST pull-down assays revealed that BcHY5 interacted with BcBBX24. Additionally, by physically binding to the promoter of BcHY5, BcBBX24 inhibited the transcriptional activity of BcHY5. Therefore, our findings reveal a transcriptional mechanism through which light response occurs in NHCC seedlings, where BcHY5 can interact with BcBBX24 and BcBBX24 can prevent BcHY5 transcription.
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