期刊
FOOD SCIENCE & NUTRITION
卷 10, 期 12, 页码 4258-4269出版社
WILEY
DOI: 10.1002/fsn3.3019
关键词
acute lung injury; amino acid metabolism; metabolomics; network pharmacology; Rosa Roxburghii Tratt fruit polyphenols; sphingolipid metabolism
资金
- Guizhou Provincial Health Commission Project [[2021]141]
- scientific research project of major agricultural industry of Guizhou province [KY[2019]009]
- social development project of Guizhou Department of Science and Technology [[2020]4Y214]
- Youth Science and technology Talents Development Project of Education Department of Guizhou Province [KY[2022]185]
This study aimed to explore the anti-ALI activity of Chinese functional food Rosa roxburghii Tratt fruit polyphenols (RRTP). The results indicated that RRTP significantly attenuated the severity of pathological changes and pulmonary capillary permeability in ALI mice. Metabolomics studies revealed that RRTP markedly affected multiple metabolites and metabolic pathways. Network pharmacology analysis identified the core active components and potential therapeutic targets of RRTP.
Acute lung injury (ALI) is a respiratory disease with high morbidity and mortality rates and is the primary cause of death in children and the elderly around the world. The use of Chinese foods in the complementary and alternative treatment of ALI has attracted more and more attention. This study aimed to explore the anti-ALI activity of Chinese functional foods Rosa roxburghii Tratt fruit polyphenols (RRTP). RRTP was administered to lipopolysaccharide-induced ALI mice, and its protective effects were comprehensively evaluated by lung histopathological examination, wet/dry (W/D) ratio, and cytokine production. Metabolomics analysis was used to identify the differential metabolites and metabolic pathways in plasma, and molecular docking and systemic biology-based network pharmacology assay were performed to explore the active components and potential therapeutic targets. The results indicated that RRTP significantly attenuated the severity of pathological changes and pulmonary capillary permeability. Furthermore, RRTP limited the increase in tumor necrosis factor alpha (TNF-alpha), interleukin 1 beta (IL-1 beta), and interleukin 6 (IL-6) levels and the decrease in interleukin 10 (IL-10) levels in ALI mice. Metabolomics studies revealed that RRTP markedly affected 19 different metabolites, three amino acid metabolism pathways, and sphingolipid metabolism. Moreover, network pharmacology identified AKT1 (AKT serine/threonine kinase 1), TP53, IL-6, VEGFA (vascular endothelial growth factor A), and TNF (tumor necrosis factor) as the most promising target proteins, while quercetin, luteolin, and kaempferol were the core active components of RRTP. This study investigated the complex mechanisms of RRTP against ALI for the first time, and provided a foundation for the application of RRTP as a functional food, facilitating the research of nutritional food additives for the adjuvant treatment of ALI.
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