Expression Profiling of Circular RNAs in Early Pregnant Jianghuai Buffaloes

Simple Summary CircRNA transcriptome sequencing technology is widely used in the study of germ-line stem cell proliferation and differentiation and early embryonic development, but the research on early pregnancy diagnosis in female animals is still in the preliminary stage, and the specific regulatory mechanism has not been reported. Here, we identified numerous differentially expressed circRNAs (DECs) in the venous blood of the distinctive local breed of Chinese Jianghuai buffalo. Analysis of the enrichment showed that DECs were mainly enriched in the epidermal growth factor receptor-signaling pathway that is important for embryonic development and pregnancy maintenance. These findings have provided a better understanding of buffalo pregnancy establishment and a potential basis for improving early pregnancy diagnosis techniques in buffalo. Circular RNA (circRNA) is expressed in cells and tissues of several species. However, the expression of circRNAs in the blood of Jianghuai buffaloes during early pregnancy has not been reported. In this study, we identified the DECs in the blood of Jianghuai buffaloes and annotated the functions of these DECs. The results showed that there were 890 DECs between the pregnant and non-pregnant groups, of which more than 80% were exon-derived circRNAs, including 323 up-regulated circRNAs and 567 down-regulated circRNAs. Enrichment analysis revealed that DECs were mainly enriched in the epidermal growth factor receptor-signaling pathway important for embryonic development and pregnancy maintenance. In addition, most DECs have multiple miRNA targets, suggesting that these DECs have the potential to function as miRNA sponges. In conclusion, several DECs are present between pregnant and non-pregnant Jianghuai buffaloes, and these DECs are associated with embryo implantation and pregnancy establishment.

Automated summary

In this study, differentially expressed circRNAs (DECs) were identified in the blood of the distinctive local breed of Chinese Jianghuai buffalo, and these DECs were mainly enriched in the epidermal growth factor receptor-signaling pathway important for embryonic development and pregnancy maintenance. These findings provide a better understanding of buffalo pregnancy establishment and a potential basis for improving early pregnancy diagnosis techniques in buffalo.