期刊
PROCESSES
卷 10, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/pr10112224
关键词
Phoenix dactylifera; date fruit flesh; date varieties; supercritical CO2; subcritical CO2; phytochemicals; biological properties
资金
- National Plan for Science, Technology and Innovation (MAARIFAH), King Abdulaziz City for Science and Technology, Kingdom of Saudi Arabia [15-AGR3527-02]
Supercritical and subcritical extraction techniques were used to produce flesh extracts from four Saudi date fruits. The subcritical CO(2) method showed significant extraction of phytochemicals, while the Majdool date variety exhibited the highest levels of both phytochemicals and biological properties.
Saudi Arabia is one of the major producers of date (Phoenix dactylifera) fruit. Date fruit flesh is considered a healthy food due to the presence of natural antioxidants. Green and innovative supercritical fluid (SFE, 52.5 degrees C temperature, 27.50 MPa pressure, 5 mL CO2/min flow rate) and subcritical (SubCO(2), 250 extraction cycles, 29 degrees C temperature, 6.8 MPa, 12 h, ethanol solvent) extraction techniques were used to produce flesh extracts from four Saudi date fruits (Sukari (SKFE), Ambara (AMFE), Majdool (MJFE) and Sagai (SGFE)), and extracts prepared using 6 h Soxhlet extraction at 70 degrees C for 16 h using n-hexane as solvent, were taken as control. SFE produced the highest (p < 0.05) extract yields, whereas the SubCO(2) method recovered significantly higher (p < 0.05) amounts of phytochemicals. Total phenolics (186.37-447.31 mg GAE/100 g), total flavonoids (82.12-215.28 mg QE/100 g), total anthocyanins (0.41-1.34 mg/100 g), and total carotenoid (1.24-2.85 mg BCE/100 g) were quantified in all the flesh extracts. The biological properties evaluation showed that flesh extracts had high antioxidant (17.79-45.08 mu g AAE/mL), antiradical (191.36-34.66 mu g/mL DPPH IC50), ferricreducing (2.18-5.01 mmol TE/100 g) and ABTS-scavenging (444.75-883.96 mu mol TE/100 g) activities. SubCO(2) was the best technique and Majdool the best date variety, in terms of both phytochemicals and biological properties.
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