期刊
PHARMACEUTICS
卷 14, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/pharmaceutics14102201
关键词
Salmonella enterica; hen egg white lysozyme; cecropin A; amphipathic helix; food preservative
资金
- Ministry of Education
- King Abdulaziz University, DSR, Jeddah, Saudi Arabia
- [IFPIP: 85-142-1442]
This study presents a safe and effective approach utilizing cecropin A to facilitate hen egg white lysozyme access to peptidoglycan layers. The combination of HEWL and cecropin A shows potential antibacterial activity against Salmonella enterica.
The prevalence of multidrug-resistant Salmonella enterica among animal- and plant-derived food products threatens global healthcare and economic sectors. Hen egg white lysozyme is widely exploited as a food preservative against Gram-positive pathogens. Nevertheless, its limited penetration of the outer membrane renders it ineffective against Gram-negative bacteria. Herein, we present a safe and effective approach to facilitate HEWL access to peptidoglycan layers using cecropin A. In silico analysis of cecropin A peptide revealed an amphipathic a-helical peptide with potential outer membrane permeabilizing activity through its interaction with both hydrophobic and ionic stabilizing forces. Evaluation of HEWL/cecropin A combination showed a cecropin A dose-dependent bacterial count reduction up to 4.16 and 3.18 +/- 0.26 log units against Salmonella enterica ATCC 35664 at the logarithmic and stationary growth phases, respectively. Moreover, the combination displayed antibacterial activity of 2.1 +/- 0.31 and similar to 1 log-unit reductions against Salmonella enterica serovars Kentucky, Typhimurium, and Enteritidis, respectively, whereas Hato and Shangani were found irresponsive. The cytotoxicity assay revealed compatibility of cecropin A with oral epithelial cells. These observations suggest HEWL/cecropin A combination as an effective and safe alternative to lysozyme against Salmonella enterica.
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