期刊
CELLS
卷 11, 期 19, 页码 -出版社
MDPI
DOI: 10.3390/cells11193070
关键词
endochondral; intramembranous; ossification; bone tissue engineering; mesenchymal stromal cells
类别
资金
- NIH [EB027062]
- City of Vienna Project [30-11]
- Whitaker Foundation
In this study, the effects of endochondral and intramembranous ossification pathways on the properties of engineered bone grafts were evaluated. The results showed that grafts generated through endochondral pathway exhibited better bone regeneration and vascularization, making it more suitable for regenerating large defects. On the other hand, the intramembranous ossification pathway could be used to guide tissue formation with scaffold architecture.
Bone grafts can be engineered by differentiating human mesenchymal stromal cells (MSCs) via the endochondral and intramembranous ossification pathways. We evaluated the effects of each pathway on the properties of engineered bone grafts and their capacity to drive bone regeneration. Bone-marrow-derived MSCs were differentiated on silk scaffolds into either hypertrophic chondrocytes (hyper) or osteoblasts (osteo) over 5 weeks of in vitro cultivation, and were implanted subcutaneously for 12 weeks. The pathways' constructs were evaluated over time with respect to gene expression, composition, histomorphology, microstructure, vascularization and biomechanics. Hypertrophic chondrocytes expressed higher levels of osteogenic genes and deposited significantly more bone mineral and proteins than the osteoblasts. Before implantation, the mineral in the hyper group was less mature than that in the osteo group. Following 12 weeks of implantation, the hyper group had increased mineral density but a similar overall mineral composition compared with the osteo group. The hyper group also displayed significantly more blood vessel infiltration than the osteo group. Both groups contained M2 macrophages, indicating bone regeneration. These data suggest that, similar to the body's repair processes, endochondral pathway might be more advantageous when regenerating large defects, whereas intramembranous ossification could be utilized to guide the tissue formation pattern with a scaffold architecture.
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