期刊
CELLS
卷 11, 期 19, 页码 -出版社
MDPI
DOI: 10.3390/cells11192932
关键词
high altitude; chronic cold exposure; pulmonary hypertension; echocardiography; microparticles
类别
资金
- Cardiovascular Medical Research and Education Fund (CMREF)
- Ministry of Education and Science of the Kyrgyz Republic [0005823]
- Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) [268555672SFB 1213 A07]
This study investigates the role of microparticles (MPs) and cold exposure in high altitude pulmonary hypertension (HAPH). The results show that long-term exposure to cold environment is associated with increased pulmonary artery pressure in high-altitude residents, and the circulating levels of MPs differ between lowlanders and HAPH highlanders during the colder season.
The role of microparticles (MPs) and cold in high altitude pulmonary hypertension (HAPH) remains unexplored. We investigated the impact of long-term cold exposure on the pulmonary circulation in lowlanders and high-altitude natives and the role of MPs. Pulmonary hemodynamics were evaluated using Doppler echocardiography at the end of the colder and warmer seasons. We further examined the miRNA content of MPs isolated from the study participants and studied their effects on human pulmonary artery smooth muscle (hPASMCs) and endothelial cells (hPAECs). Long-term exposure to cold environment was associated with an enhanced pulmonary artery pressure in highlanders. Plasma levels of CD62E-positive and CD68-positive MPs increased in response to cold in lowlanders and HAPH highlanders. The miRNA-210 expression contained in MPs differentially changed in response to cold in lowlanders and highlanders. MPs isolated from lowlanders and highlanders increased proliferation and reduced apoptosis of hPASMCs. Further, MPs isolated from warm-exposed HAPH highlanders and cold-exposed highlanders exerted the most pronounced effects on VEGF expression in hPAECs. We demonstrated that prolonged exposure to cold is associated with elevated pulmonary artery pressures, which are most pronounced in high-altitude residents. Further, the numbers of circulating MPs are differentially increased in lowlanders and HAPH highlanders during the colder season.
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