4.6 Article

The Effect of Germanium-Loaded Hydroxyapatite Biomaterials on Bone Marrow Mesenchymal Stem Cells Growth

期刊

CELLS
卷 11, 期 19, 页码 -

出版社

MDPI
DOI: 10.3390/cells11192993

关键词

hydroxyapatite; germanium; drug release; mesenchymal stem cells; protein adhesion

资金

  1. MCIN/AEI [PID2020116693RB-C21, PID2020-116693RB-C22]
  2. Generalitat Valenciana [CIAICO/2021/157]
  3. Universidad Catolica San Antonio de Murcia (UCAM), Murcia, Spain [PMAFI-27/21]

向作者/读者索取更多资源

Hydroxyapatite (HA) is a mineral component used for repair and regeneration of mineralized tissues. The addition of germanium (Ge) improves the properties of HA and shows biocompatibility with BM-MSCs.
Hydroxyapatite (HA) is a hard mineral component of mineralized tissues, mainly composed of calcium and phosphate. Due to its bioavailability, HA is potentially used for the repair and regeneration of mineralized tissues. For this purpose, the properties of HA are significantly improved by adding natural and synthetic materials. In this sense, the germanium (Ge) mineral was loaded in HA biomaterial by cold isostatic pressure for the first time and characterization and biocompatibility using bone marrow mesenchymal stem cells (BM-MSCs) were investigated. The addition of Ge at 5% improved the solubility (3.32%), stiffness (18.34 MPa), water holding (31.27%) and biodegradation (21.87%) properties of HA, compared to control. Compared to all composite biomaterials, the drug-releasing behavior of HA-3% Ge was higher at pH 1 and 3 and the maximum drug release was obtained at pH 7 and 9 with HA-5% Ge biomaterials. Among the different mediums tested, the DMEM-medium showed a higher drug release rate, especially at 60 min. HA-Ge biomaterials showed better protein adhesion and apatite layer formation, which ultimately proves the compatibility in BM-MSCs culture. Except for higher concentrations of HA (5 and 10 mg/mL), the different concentrations of Ge and HA and wells coated with 1% of HA-1% Ge had higher BM-MSCs growth than control. All these findings concluded that the fabricated HA biomaterials loaded with Ge could be the potential biomaterial for culturing mammalian cells towards mineralized tissue repair and regeneration.

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