期刊
CELLS
卷 11, 期 20, 页码 -出版社
MDPI
DOI: 10.3390/cells11203266
关键词
cell fusion reprogramming; extended pluripotency; totipotency; metabolism; embryonic; extraembryonic
类别
资金
- National Research Foundation of Korea (NRF) - Korea government (MSIT) of the Republic of Korea [2020R1A2C3007562]
- Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) - Ministry of Agriculture, Food and Rural Affairs (MAFRA) [32200605-1-CG000]
- National Research Foundation of Korea [2020R1A2C3007562] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
This study demonstrates the ability of extended pluripotent stem cells (EPSCs) to transfer their extended pluripotency directly to somatic cells through cell fusion. By fusing EPSCs with neural stem cells (NSCs), researchers observed fused cells with upregulated pluripotency markers and EPSC-specific gene expression, as well as contributions to extraembryonic and embryonic lineages both in vivo and in vitro.
As a novel cell type from eight-cell-stage embryos, extended pluripotent stem cells (EPSCs) are known for diverse differentiation potency in both extraembryonic and embryonic lineages, suggesting new possibilities as a developmental research model. Although various features of EPSCs have been defined, their ability to directly transfer extended pluripotency to differentiated somatic cells by cell fusion remains to be elucidated. Here, we derived EPSCs from eight-cell mouse embryos and confirmed their extended pluripotency at the molecular level and extraembryonic differentiation ability. Then, they were fused with OG2(+/-) ROSA(+/-) neural stem cells (NSCs) by the polyethylene-glycol (PEG)-mediated method and further analyzed. The resulting fused hybrid cells exhibited pluripotential markers with upregulated EPSC-specific gene expression. Furthermore, the hybrid cells contributed to the extraembryonic and embryonic lineages in vivo and in vitro. RNA sequencing analysis confirmed that the hybrid cells showed distinct global expression patterns resembling EPSCs without parental expression of NSC markers, indicating the complete acquisition of extended pluripotency and the erasure of the somatic memory of NSCs. Furthermore, ultrastructural observation and metabolic analysis confirmed that the hybrid cells rearranged the mitochondrial morphology and bivalent metabolic profile to those of EPSCs. In conclusion, the extended pluripotency of EPSCs could be transferred to somatic cells through fusion-induced reprogramming.
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