期刊
CELLS
卷 11, 期 18, 页码 -出版社
MDPI
DOI: 10.3390/cells11182865
关键词
dicalcium phosphate dihydrate; polysaccharides; hybrid bioactive materials; critical-sized bone defect; bone volume augmentation; multi-scale characterization
类别
资金
- PHC Ulysses
- SFR Cap Sante [47249QL]
The functionalization of bone-side collagen membrane with dicalcium phosphate dihydrate, chitosan, and hyaluronic acid led to efficient repair of critical-sized bone defects in a rat model, with the formation of new bone exhibiting physiological matrix composition and structural organization. Thorough multi-scale characterization is crucial in assessing biomaterial outcomes in animal models.
In craniofacial bone defects, the promotion of bone volume augmentation remains a challenge. Finding strategies for bone regeneration such as combining resorbable minerals with organic polymers would contribute to solving the bone volume roadblock. Here, dicalcium phosphate dihydrate, chitosan and hyaluronic acid were used to functionalize a bone-side collagen membrane. Despite an increase in the release of inflammatory mediators by human circulating monocytes, the in vivo implantation of the functionalized membrane allowed the repair of a critical-sized defect in a calvaria rat model with de novo bone exhibiting physiological matrix composition and structural organization. Microtomography, histological and Raman analysis combined with nanoindentation testing revealed an increase in bone volume in the presence of the functionalized membrane and the formation of woven bone after eight weeks of implantation; these data showed the potential of dicalcium phosphate dihydrate, chitosan and hyaluronic acid to induce an efficient repair of critical-sized bone defects and establish the importance of thorough multi-scale characterization in assessing biomaterial outcomes in animal models.
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