4.6 Article

The Flavone Cirsiliol from Salvia x jamensis Binds the F1 Moiety of ATP Synthase, Modulating Free Radical Production

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CELLS
卷 11, 期 19, 页码 -

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MDPI
DOI: 10.3390/cells11193169

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F1Fo-ATP synthase; cirsiliol; light; oxidative phosphorylation; quercetin; respiratory chain complexes; resveratrol; rod outer segment; transmission electron microscopy

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This study investigated the potential of the flavonoid cirsiliol in modulating oxidative phosphorylation and reactive oxygen species production in mammalian retinal rod outer segments (OS). The results showed that cirsiliol inhibited ATP production and reduced free radical production in the OS exposed to ambient light, suggesting its potential as a promising compound against oxidative stress.
Several studies have shown that mammalian retinal rod outer segments (OS) are peculiar structures devoid of mitochondria, characterized by ectopic expression of the molecular machinery for oxidative phosphorylation. Such ectopic aerobic metabolism would provide the chemical energy for the phototransduction taking place in the OS. Natural polyphenols include a large variety of molecules having pleiotropic effects, ranging from anti-inflammatory to antioxidant and others. Our goal in the present study was to investigate the potential of the flavonoid cirsiliol, a trihydroxy-6,7-dimethoxyflavone extracted from Salvia x jamensis, in modulating reactive oxygen species production by the ectopic oxidative phosphorylation taking place in the OS. Our molecular docking analysis identified cirsiliol binding sites inside the F1 moiety of the nanomotor F1Fo-ATP synthase. The experimental approach was based on luminometry, spectrophotometry and cytofluorimetry to evaluate ATP synthesis, respiratory chain complex activity and H2O2 production, respectively. The results showed significant dose-dependent inhibition of ATP production by cirsiliol. Moreover, cirsiliol was effective in reducing the free radical production by the OS exposed to ambient light. We report a considerable protective effect of cirsiliol on the structural stability of rod OS, suggesting it may be considered a promising compound against oxidative stress.

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