Occupational Exposure to Pesticides Affects Pivotal Immunologic Anti-Tumor Responses in Breast Cancer Women from the Intermediate Risk of Recurrence and Death

Simple Summary This study presents information regarding the immunological changes induced by pesticide exposure in patients diagnosed with breast cancer occupationally exposed to pesticides. Such changes are helpful to understand tumor behavior under pesticide exposure and can be beneficial to re-stratify breast cancer patients occupationally exposed concerning their risk of disease recurrence and death. Breast cancer risk stratification is a strategy based using on clinical parameters to predict patients' risk of recurrence or death, categorized as low, intermediate, or high risk. Both low and high risk are based on well-defined clinical parameters. However, the intermediate risk depends on more malleable parameters. It means an increased possibility for either suboptimal treatment, leading to disease recurrence, or systemic damage due to drug overload toxicity. Therefore, identifying new factors that help to characterize better the intermediate-risk stratification, such as environmental exposures, is necessary. For this purpose, we evaluated the impact of occupational exposure to pesticides on the systemic profile of cytokines (IL-12, IL-4, IL-17A, and TNF-alpha) and oxidative stress (hydroperoxides, total antioxidants, and nitric oxide metabolites), as well as TGF-beta 1, CTLA-4, CD8, and CD4 expression, investigated in tumor cells. Occupational exposure to pesticides decreased the levels of IL-12 and significantly increased the expression of TGF-beta 1 and CTLA-4 in the immune infiltrate. Nevertheless, we observed a decrease in CTLA-4 in tumor samples and CD8 in infiltrating cells of intermediate overweight or obese patients with at least one metastatic lymph node at the diagnosis. These findings indicate that occupational exposure to pesticides changes the molecular behavior of disease and should be considered for intermediate-risk stratification assessment in breast cancer patients.

Automated summary

This study examines the immunological changes induced by pesticide exposure in breast cancer patients and finds that occupational exposure to pesticides alters the molecular behavior of the disease, which should be considered in risk assessment for breast cancer patients.