期刊
FOOD BIOSCIENCE
卷 50, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.fbio.2022.102138
关键词
Skipjack tuna(Katsuwonuspelamis); Skin gelatin; Peptide; Antioxidant activity; Cytoprotection; Ultraviolet-A injury
资金
- National Natural Science Foundation of China
- Ten-thousand Talents Plan of Zhejiang Province
- [82073764]
- [2019R52026]
Gelatins (STG) were extracted from skipjack tuna skins using acid, enzyme, and hot water methods. Acid extracted gelatin (STG-A) showed the highest transparency and gel strength. STG-AH, a neutrase hydrolysate of STG-A, exhibited the highest antioxidant activity and could protect human skin fibroblasts against UVA injury. Furthermore, nineteen peptides with high antioxidant activity were identified from STG-AH.
For full use of Skipjack tuna (Katsuwonus pelamis) canning by-products, gelatins (STG) were extracted from tuna skins using acid (STG-A), enzyme (STG-E) and hot water (STG-H) methods with yields of 65.30 +/- 1.56%, 39.83 +/- 1.61%, and 50.97 +/- 1.44%, respectively. Due to the high imino acid contents (228, 216, and 213 residues/ 1000 residues for STG-A, STG-E, and STG-H, respectively), STG-A showed the highest transparency and gel strength properties. Amino acid analysis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), ultraviolet (UV) and Fourier transform infrared spectroscopy (FTIR) analysis confirmed that STG-A, STG-E, and STG-H kept the main structure of type I collagen, but enzyme and hot water extraction methods showed much stronger hydrolysis ability on alpha and beta chains. Moreover, neutrase hydrolysate (STG-AH) of STG-A showed the highest 1,1-diphenyl-2-picrylhydrazyl radical (DPPH center dot) scavenging activity and the fraction STG-AH-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I (<3.5 kDa) from STG-AH prepared by ultrafiltration could significantly protect human skin fibroblasts (HSFBs) against ultraviolet-A (UVA) injury. Furthermore, nineteen peptides with high antioxidant activity were purified from STG-AH-CYRILLIC CAPITAL LETTER BYELORUSSIAN-UKRAINIAN I and identified. These results suggested that STG-AH and its derived antioxidant peptides could serve as potential ingredients applied in health benefiting products for preventing UVA injury.
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