Unveiling the potentials of bioactive oligosaccharide1-kestose (GF2) from Musa paradisiaca Linn peel with an anxiolytic effect based on gut microbiota modulation in stressed mice model

Prebiotics, indigestible oligosaccharides found primarily in plant bi-product, aided probiotic development. Banana peels were used as cereal by-products in this study. The possibility of using them as an antistress prebiotics efficacy was examined through in-vitro and in-vivo analysis. Fructo-oligosaccharides (1-kestose (GF2)) in Bannan peel oligosaccharide enzymatic extract (BPOE) were purified, further prebiotic effectiveness was analyzed and the anti-stress effect was compared in mice. In addition, Caenorhabditis elegans and fibroblasts cell line on prebiotic efficacy and cytotoxicity was determined. As a result of enzymatic treatment, kestose (GF2) was quantified using HPLC-RID, HSCC Chromatography at 24.9 mg/g, and COZY confirmed the structural. Further, the oligosaccharide enriched cookies enhanced the growth of Lactobacillus rhamnosus. Additionally, the in-vivo result indicates that the kestose fed mice reduced the stress and significantly decreased corticosterone levels, mainly due to changes in microbiota, such as an increase in the abundance of Oscilospira, Mucisprillum, and Parabactoroides species likewise decrease in Clostridium, coprobacillus, and Sporosarona species, correspondingly the behaviour change was confirmed for anxiolytic effect. This framework has the potential to advance the treatment of clinical anxiety.

Automated summary

This study found that fructo-oligosaccharides in banana peels had prebiotic effects and could be used as an antistress agent. In vitro and in vivo analysis showed that consumption of these oligosaccharides reduced stress and corticosterone levels, indicating an anxiolytic effect.